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Methods Fungal strains, culture conditions, and chemical treatments Wild-type L

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Theres an angiogenic pathway that it probably works through, and an antioxidant pathway that it probably works through. Angiogenesis, he explains, is a fancy way of saying it grows new vessels

14 PCR amplification was performed in a final volume of 25 l of the reaction mixture containing 1.5 U of Taq DNA polymerase (Sibenzyme), about 1 g DNA of each patient's DNA, 0.25 M each primer, 250 M of dNTPs, 2 mM of MgCl 2 , 1 PCR buffer of the following composition: 67 mM Tris-HCl pH 8.8, 16.6 mM (NH 4 ) 2 SO 4 and 0.01% Tween-20